Preparation Instructions

The following English translations are based on both EU and US FDA guidelines for safe baby formula preparation:
- Wash hands and sterilize bottles, nipples, and accessories.
- Boil fresh water. Cool to ~40–50°C as per label guidance.
- Add the exact number of level 4.4g scoops.
- Close bottle and shake until powder fully dissolves.
- Cool under running water to ~37°C (~98°F). Test before feeding.
- Discard any unfinished formula within 1 hour.
Periodic packaging updates are common. Always follow the preparation and storage directions printed on the product label.
Safe Storage Instructions
- Mixed formula should be consumed within an hour in order to prevent bacteria from growing in the prepared formula milk.
- Once opened, the manufacturer recommends to use the box of formula within 21 days. Discard any unusued formula powder if the box hasn't been used up yet.
- Each box should be stored in a low-light, cool temperature environment out of direct sunlight.
- It isn't necessary to refrigerate the unmixed box of formula.
- To prevent uneven heating, it is recommended to pour heated water into a bottle, rather than microwaving water that is already inside the bottle.
Feeding Chart
Please note that there are multiple styles of Holle Bio Stage 1 boxes and you may receive either.
Age | Water | Scoops | Ready-Made Formula | Meals Per Day | Boxes Needed |
---|---|---|---|---|---|
1-2 w. | Consult your pediatrician | ||||
2-4 w. | 90ml (3 oz) | 3 | 100ml (3.4 oz) | 5-7 | About 3 |
2-3 m. | 150ml (5.1 oz) | 5 | 170ml (5.7 oz) | 4-5 | About 12 |
3-4 m. | 180ml (6 oz) | 6 | 200ml (6.8 oz) | 4-5 | About 14.5 |
5+ m. | 210ml (7.1 oz) | 7 | 230ml (7.8 oz) | 3-4 | 8.5 per month |
For babies aged above 6 months, it is advisable to prepare Holle formula for an older baby and transition up a stage to the next formula (Holle Bio Stage 2) which offers age-appropriate nutrition.
If you are mixing Holle formula but your baby has already started to transition to purees or solids, it is common to replace 1 daily bottle for 1 full jar of puree or 1 full portion of solids.
If your baby does not finish a full bottle once it is prepared, most parents will still count the feed towards the daily number of total feeds. Feeding amounts and daily servings are general guidelines. Every baby’s appetite is different, so adjust as needed while maintaining a balanced diet and healthy routine.
The above preparation instructions for Holle Bio Stage 1 Formula lists details found on the most recent packaging style. However, these mixing instructions may be updated at any time by Holle, so double check your box to see if you may have an even newer packaging style.
Ingredients
As listed on the packaging. Scroll further for interactive ingredients mapping.
Ingredient certifications:
- European Organic
- Demeter certified for sustainable farming practices

Simplified Ingredients
Hover over any ingredient to see its category.
Holle Stage 1 Organic Nutritional Facts
(Per 100ml or 3.38 oz/3 oz prepared)
- Energy 68 kcal
- Total Fat 3.4 g
-
- Saturates 1.0 g
- Monounsaturates 1.8 g
- Polyunsaturates 0.6 g
- Linoleic acid 546 mg
- Linolenic Acid (Omega 3) 54 mg
- DHA 15 mg
- ARA 15 mg
- Total Carbs 8.0 g
-
- Sugars 8.0 g
- Lactose 8.0 g
- Inositol 4.6 mg
- Protein 1.4 g
- L-Carnitine 2.2 mg
- Sodium 24 mg
- Potassium 82.2 mg
- Calcium 59.9 mg
- Iron 0.54 mg
- Phosphorus 35.9 mg
- Magnesium 5.40 mg
- Chloride 50.9 mg
- Zinc 0.45 mg
- Copper 0.055 mg
- Iodine 14.2 mcg
- Manganese 0.0053 mg
- Selenium 3.9 mcg
- Fluoride 0.010 mg
- Vitamin C 6.1 mg
- Vitamin A 54.8 mcg
- Vitamin D 1.5 mcg
- Vitamin E 1.1 mg
- Vitamin B1 0.035 mg
- Vitamin B2 0.18 mg
- Vitamin B6 0.034 mg
- Vitamin B12 0.14 mcg
- Folic Acid 10.1 mcg
- Folate 16.9 mcg DFE
- Pantothenic Acid 0.35 mg
- Vitamin K 5.4 mcg
- Biotin 1.6 mcg
- Niacin 0.35 mg
- Choline 23 mg